含IQ基元GTP酶激活蛋白1(IQGAP1)重组蛋白

YB287Mu021
Recombinant IQ Motif Containing GTPase Activating Protein 1 (IQGAP1)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
含IQ基元GTP酶激活蛋白1(IQGAP1)重组蛋白[ PROPERTIES ]
Residues: Gln1276~Lys1657
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q9JKF1
Host: E. coli
Subcellular Location: Cell membrane.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
含IQ基元GTP酶激活蛋白1(IQGAP1)重组蛋白pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 7.8
Predicted Molecular Mass: 47.5kDa
Accurate Molecular Mass: 58kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
含IQ基元GTP酶激活蛋白1(IQGAP1)重组蛋白protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
QDKFN VDEYSDLVTL TKPVIYISIG EIINTHTLLL DHQDAIAPEH NDPIHELLDD LGEVPTIESL
IGESCGNSND PNKEALAKTE VSLTLTNKFD VPGDENAEMD ARTILLNTKR LIVDVIRFQP
GETLTEILET PATNEQEAEH QRAMQRRAIR DAKTPDKMKK SKPMKEDNNL SLQEKKEKIQ
TGLKKLTELG TVDPKNRYQE LINDIAKDIR NQRRYRQRRK AELVKLQQTY SALNSKATFY
GEQVDYYKSY IKTCLDNLAS KGKVSKKPRE MKGKKSKKIS LKYTAARLHE KGVLLEIEDL
QANQFKNVIF EIGPTEEVGD FEVKAKFMGV QMETFMLHYQ DLLQLQYEGV AVMKLFDRAK
VNVNLLIFLL NKKFYGK