AMPA离子能谷氨酸受体2(GRIA2)重组蛋白

YB802Mu021
Recombinant Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 AMPA离子能谷氨酸受体2(GRIA2)重组蛋白[ PROPERTIES ]
Residues: Asn25~Ala543
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P42262
Host: E. coli
Subcellular Location: Cell membrane, Multi-pass
membrane protein, Endoplasmic reticulum
membrane, Cell junction, synapse, postsynaptic cell
membrane.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
 AMPA离子能谷氨酸受体2(GRIA2)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.2
Predicted Molecular Mass: 62.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
NSIQIG GLFPRGADQE YSAFRVGMVQ FSTSEFRLTP HIDNLEVANS FAVTNAFCSQ
FSRGVYAIFG FYDKKSVNTI TSFCGTLHVS FITPSFPTDG THPFVIQMRP DLKGALLSLI
 AMPA离子能谷氨酸受体2(GRIA2)重组蛋白EYYQWDKFAY LYDSDRGLST LQAVLDSAAE KKWQVTAINV GNINNDKKDE MYRSLFQDLE
LKKERRVILD CERDKVNDIV DQVITIGKHV KGYHYIIANL GFTDGDLLKI QFGGANVSGF
QIVDYDDSLV SKFIERWSTL EEKEYPGAHT TTIKYTSALT YDAVQVMTEA FRNLRKQRIE
ISRRGNAGDC LANPAVPWGQ GVEIERALKQ VQVEGLSGNI KFDQNGKRIN YTINIMELKT
NGPRKIGYWS EVDKMVVTLT ELPSGNDTSG LENKTVVVTT ILESPYVMMK KNHEMLEGNE
RYEGYCVDLA AEIAKHCGFK YKLTIVGDGK YGARDADTKI WNGMVGELVY GKADIAIAPL
TITLVREEVI DFSKPFMSLG ISIMIKKPQK SKPGVFSFLD PLA