恒河猴糖化血红蛋白A1c(HbA1c)多克隆抗体
Polyclonal Antibody to Glycated Hemoglobin A1c (HbA1c)
[ PRODUCT INFORMATION ]
Immunogen: HbA1C
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Native Protein.
[ RELEVANCE ]
The HbA1c shows the average amount of glucose in the blood over a period of 3months. Sugar in the bloodstream can become attached to the hemoglobin in redblood cells (glycosylation). Once the sugar is attached, it stays there for the life ofthe red blood cell, which is about 120 days. The higher the level of blood sugar,the more sugar attaches to red blood cells. The HbA1c is formed in anon-enzymatic pathway by hemoglobin's standard exposure to elevated plasmalevels of glucose. HbA1c is tested to monitor nephropathy and retinopathy indiabetes mellitus.
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against HbA1C. It has beenselected for its ability to recognize HbA1C in immunohistochemical staining andwestern blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[CONTENTS ] 
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN₃, 50%glycerol.
[STORAGE ] 
Store at 4 °C for frequent use. Stored at -20°C to -80 °C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.