PEX14 抗体Applications
| In-house applications |
ELISA, WB, IP, IHC, FC, IF Application not tested? Please see our Guarantee. |
| Published applications |
IF, IHC, WB |
Publications |
12 citations –view here |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
| Published Species |
Human, monkey, mouse, rat |
|
Images
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 human liver tissue were subjected to SDS PAGE followed by western blot with 10594-1-AP(PEX14 antibody) at dilution of 1:500 IF result of anti-PEX14 (10594-1-AP) in Hela cell by Dr. Sven Thoms. Peroxisome Red,Nuclear Blue. Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 10594-1-AP( PEX14 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) Immunohistochemical of paraffin-embedded human liver cancer using 10594-1-AP(PEX14 antibody) at dilution of 1:50 (under 10x lens) Immunohistochemical of paraffin-embedded human liver cancer using 10594-1-AP(PEX14 antibody) at dilution of 1:50 (under 40x lens) IP Result of anti-PEX14 (IP:10594-1-AP, 3ug; Detection:10594-1-AP 1:500) with mouse liver tissue lysate 4000ug. 1X10^6 HepG2 cells were stained with .2ug PEX14 antibody (10594-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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| Positive WB detected in |
Human liver tissue |
| Positive IP detected in |
Mouse liver tissue |
| Positive IHC detected in |
Human liver cancer tissue, human cervical cancer tissue |
| Positive IF detected in |
HepG2 cells, COS7 cells, HeLa cells |
| Positive FC detected in |
HepG2 cells |
| Recommended dilution |
WB : 1:200-1:1000
IP : 1:200-1:2000
IHC : 1:50-1:200
IF : 1:50-1:500 |
PEX14 抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen |
PEX14 fusion protein ag0932 |
Full name |
Peroxisomal biogenesis factor 14 |
Calculated molecular
weight |
41 kDa |
Observed molecular
weight |
57 kDa |
GenBank accession
number |
BC006327 |
Gene ID (NCBI) |
5195 |
| Gene symbol |
PEX14 |
Synonyms |
DJ734G22.2, NAPP2, Peroxin 14, PEX14, Pex14p, PTS1 receptor docking protein |
Background PEX14 抗体
|
PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells. PEX14 抗体
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