KSP钙黏蛋白(CDH16)重组蛋白

YB998Hu012
Recombinant Cadherin 16 (CDH16)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]
Residues: Phe37~Asn287 (Accession # O75309),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Cell membrane;
Single-pass type I membrane protein.
 KSP钙黏蛋白(CDH16)重组蛋白Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 5.6
Predicted Molecular Mass: 31.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
 KSP钙黏蛋白(CDH16)重组蛋白that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
 KSP钙黏蛋白(CDH16)重组蛋白sequence is listed below.
MGSSHHHHHH SSG LVPRGSH MASMTGGQQM GRGSEF - FPLY LTKLPLPREG
AEGQIVLSGD SGKATEGPFA MDPDSGFLLV TRALDREEQA EYQLQVTLEM QDGHVLWGPQ
PVLVHVKDEN DQVPHFSQAI YRARLSRGTR PGIPFLFLEA SDRDEPGTAN SDLRFHILSQ
APAQPSPDMF QLEPRLGALA LSPKGSTSLD HALERTYQLL VQVKDMGDQA SGHQATATVE
VSIIESTWVS LEPIHLAENL KVLYPHHMAQ VHWSGGDVHY HLESHPPGPF EVNAEGN