人胰腺癌细胞
D/F12+5%FBS+0.002mg/ml胰岛素+0.005mg/ml转铁蛋白+40ng/ml氢化可的松+10ng/ml表皮生长因子EGF +1%P/S
人真皮成纤维细胞(原代细胞永生化)
D/F12+10%FBS+1%p/s+0.005mg/ml胰岛素+5ng/mlBfGF+1ug/ml氢化可的松+50ug/ml抗坏血酸(维C)+7.5mM L-Gln (推荐:PriMed-iCell-003)
人胰腺癌细胞
D/F12+5%FBS+0.002mg/ml胰岛素+0.005mg/ml转铁蛋白+40ng/ml氢化可的松+10ng/ml表皮生长因子EGF +1%P/S
人真皮成纤维细胞(原代细胞永生化)
D/F12+10%FBS+1%p/s+0.005mg/ml胰岛素+5ng/mlBfGF+1ug/ml氢化可的松+50ug/ml抗坏血酸(维C)+7.5mM L-Gln (推荐:PriMed-iCell-003)